Publication Date: Mon Mar 29 00:00: Research Org.: Lawrence Livermore National Lab.
Johns Hopkins Univ., Baltimore, MD (United States).
Columbia Univ., New York, NY (United States).The Scripps Research Inst., La Jolla, CA (United States).National Institutes of Health (NIH), Bethesda, MD (United States).Jude Children's Research Hospital, Memphis, TN (United States) Heinrich-Heine-Univ., Düsseldorf (Germany).To make further progress, we strongly encourage ‘open science’ practices. This position paper describes the current ‘state of the art’ from different perspectives, points to unresolved methodological issues for quantitative structural studies, provides a set of ‘soft recommendations’ about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. These multi-lab studies have led to the development of smFRET procedures and documentation, which are important when submitting entries into the archiving system for integrative structure models, PDB-Dev. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics.
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